Impact of copper on protein pattern
of coelomic fluid in red earthworm
A. Zebarjad 1, M. Arabi 1, A.M. Ahadi 2
Biology Depat., 2 Genetics Dept.; Faculty of Basic Sciences, Shahrekord
Copper ions are well known stressors in both invertebrates
and vertebrates. Generally, negative impact of copper was always seen in animal
models. Earthworms are one of the best models in immune-toxicological studies
as they are abundant and have a unique immune system. In the body extract of
them, there is a glycolipoprotein compound viz. G-90 complex (20-70 KDa fractions) that
possesses antioxidant, anti-bacterial and mitotic characteristics. It has been
shown that G-90 may applied as a medicine to cure some disease particularly in wound
healing. In present investigation, red earthworms (Lumbricus rubellus;
Lumbricidae) were used as animal model which treated with copper ions as
a physico-chemical stressor.
In our study, copper sulfate (LD50 = 150 mg/Kg
soil) in different concentrations 25, 50 and 100 mg/Kg artificial soil were
used for 14 days under constant laboratory conditions. SDS-PAGE electrophoresis technique applied to
show possible changes in the coelomic protein pattern for fractions of G-90
complex and also FRAP (ferric reducing antioxidant power) values were used to
measure antioxidant power in red earthworm Lumbricus rubellus coelomic fluid.
Our electrophoretic results (using Image J software) showed
that in treated groups with copper, the intensity of 20-70 KDa gel bands was
increased compared to controls indicating elevation in gene expression of G-90
fractions. FRAP values showed that copper treatment caused an increase in
antioxidant power of coelomic fluid to encounter unwanted and abnormal
situations e.g. copper stress.
Collectively, copper sulfate stress can potentiate the antioxidant
power and also increase the amount of different fractions of G-90 complex in earthworm
body under laboratory conditions. Therefore, copper stress can be used as an
agent to increase the power of G-90 complex in cure of different diseases.
Keyword: Copper, Red earthworm, Coelomic
fluid, G-90, Protein pattern, FRAP.
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